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gdna yield dna concentrations  (Thermo Fisher)


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    Thermo Fisher gdna yield dna concentrations
    Flow chart depicting steps to assemble the filtration units and the methodology to be followed for isolating <t>gDNA</t> using the PES membrane. Note that the methodology described requires no centrifugation steps. RT, room temperature; TE, Tris-EDTA.
    Gdna Yield Dna Concentrations, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gdna+yield+dna+concentrations/DNA/pmc05865945-76-3-13
    Average 99 stars, based on 1 article reviews
    gdna yield dna concentrations - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood"

    Article Title: Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood

    Journal: Journal of Biomolecular Techniques : JBT

    doi: 10.7171/jbt.18-2902-001

    Flow chart depicting steps to assemble the filtration units and the methodology to be followed for isolating gDNA using the PES membrane. Note that the methodology described requires no centrifugation steps. RT, room temperature; TE, Tris-EDTA.
    Figure Legend Snippet: Flow chart depicting steps to assemble the filtration units and the methodology to be followed for isolating gDNA using the PES membrane. Note that the methodology described requires no centrifugation steps. RT, room temperature; TE, Tris-EDTA.

    Techniques Used: Filtration, Membrane, Centrifugation

    Agarose gel electrophoresis of gDNA samples prepared using different PES membranes or the QIAamp commercial kit. A) Buffy coat from the same blood sample was equally distributed and processed for gDNA extraction using either 0.22 µm PES membrane from EMD Millipore (lane 1) or QIAamp DNA Blood Mini Kit from Qiagen (lane 2). B) Buffy coat from different samples was lysed independently, pooled together, and equally distributed and processed for gDNA extraction using 0.22 µm PES membrane from different suppliers (lane 1: EMD Millipore; lane 2: Pall; and lane 3: Sterlitech). C) Buffy coat from the same sample was equally distributed and processed for gDNA extraction using either 0.22 µm (lane 1) or 0.45 µm (lane 2) PES membrane from EMD Millipore. M, DNA MW marker (75 bp–20 kb). Representative of a minimum of 4 independent experiments.
    Figure Legend Snippet: Agarose gel electrophoresis of gDNA samples prepared using different PES membranes or the QIAamp commercial kit. A) Buffy coat from the same blood sample was equally distributed and processed for gDNA extraction using either 0.22 µm PES membrane from EMD Millipore (lane 1) or QIAamp DNA Blood Mini Kit from Qiagen (lane 2). B) Buffy coat from different samples was lysed independently, pooled together, and equally distributed and processed for gDNA extraction using 0.22 µm PES membrane from different suppliers (lane 1: EMD Millipore; lane 2: Pall; and lane 3: Sterlitech). C) Buffy coat from the same sample was equally distributed and processed for gDNA extraction using either 0.22 µm (lane 1) or 0.45 µm (lane 2) PES membrane from EMD Millipore. M, DNA MW marker (75 bp–20 kb). Representative of a minimum of 4 independent experiments.

    Techniques Used: Agarose Gel Electrophoresis, Extraction, Membrane, Marker

    PCR of different HLA targets using gDNA prepared using the PES membrane or QIAamp kit. Buffy coat from the same blood sample was equally distributed and processed for gDNA extraction using either 0.22 µm PES membrane from EMD Millipore (A) or QIAamp DNA Blood Mini Kit from Qiagen (B). Different targets of HLA loci were PCR amplified, as described in Validation of the gDNA isolation method using the PES membrane filter. PCR amplicons were analyzed on 2.5% agarose gel electrophoresis. Representative of a minimum of 4 independent experiments. Lanes 1 and 2: HLA-A Exons 2 and 3; lanes 3 and 4: HLA-B Exons 2 and 3; lanes 5 and 6: HLA-C Exons 2 and 3; lanes 7 and 10: HLA-DPB1 Exons 3 and 2, respectively; lanes 8 and 11: HLA-DQB1 Exons 3 and 2, respectively; lanes 9 and 12: HLA-DRB1 Exons 3 and 2, respectively. M, DNA MW marker (250 bp–10 kb).
    Figure Legend Snippet: PCR of different HLA targets using gDNA prepared using the PES membrane or QIAamp kit. Buffy coat from the same blood sample was equally distributed and processed for gDNA extraction using either 0.22 µm PES membrane from EMD Millipore (A) or QIAamp DNA Blood Mini Kit from Qiagen (B). Different targets of HLA loci were PCR amplified, as described in Validation of the gDNA isolation method using the PES membrane filter. PCR amplicons were analyzed on 2.5% agarose gel electrophoresis. Representative of a minimum of 4 independent experiments. Lanes 1 and 2: HLA-A Exons 2 and 3; lanes 3 and 4: HLA-B Exons 2 and 3; lanes 5 and 6: HLA-C Exons 2 and 3; lanes 7 and 10: HLA-DPB1 Exons 3 and 2, respectively; lanes 8 and 11: HLA-DQB1 Exons 3 and 2, respectively; lanes 9 and 12: HLA-DRB1 Exons 3 and 2, respectively. M, DNA MW marker (250 bp–10 kb).

    Techniques Used: Membrane, Extraction, Amplification, Biomarker Discovery, Isolation, Agarose Gel Electrophoresis, Marker

    PCR of different HLA targets using gDNA prepared using PES membranes from different suppliers. Buffy coat from different samples was lysed independently, pooled together, and equally distributed and processed for gDNA extraction using the 0.22 µm PES membrane from different suppliers: EMD Millipore (A), Pall (B), and Sterlitech (C). Different targets of HLA loci were PCR amplified, as described in Validation of the gDNA isolation method using the PES membrane filter. PCR amplicons were analyzed on 2.5% agarose gel electrophoresis. Representative of a minimum of 4 independent experiments. Lane 1: HLA-A Exon 2; lane 2: HLA-A Exon 3; lane 3: HLA-B Exon 2; lane 4: HLA-B Exon 3; lane 5: HLA-C Exon 2; lane 6: HLA-C Exon 3; lane 7: HLA-DPB1 Exon 3; lane 8: HLA-DQB1 Exon 3; lane 9: HLA-DRB1 Exon 3; lane 10: HLA-DPB1 Exon 2; lane 11: HLA-DQB1 Exon 2; lane 12: HLA-DRB1 Exon 2. M, DNA MW marker (250 bp–10 kb).
    Figure Legend Snippet: PCR of different HLA targets using gDNA prepared using PES membranes from different suppliers. Buffy coat from different samples was lysed independently, pooled together, and equally distributed and processed for gDNA extraction using the 0.22 µm PES membrane from different suppliers: EMD Millipore (A), Pall (B), and Sterlitech (C). Different targets of HLA loci were PCR amplified, as described in Validation of the gDNA isolation method using the PES membrane filter. PCR amplicons were analyzed on 2.5% agarose gel electrophoresis. Representative of a minimum of 4 independent experiments. Lane 1: HLA-A Exon 2; lane 2: HLA-A Exon 3; lane 3: HLA-B Exon 2; lane 4: HLA-B Exon 3; lane 5: HLA-C Exon 2; lane 6: HLA-C Exon 3; lane 7: HLA-DPB1 Exon 3; lane 8: HLA-DQB1 Exon 3; lane 9: HLA-DRB1 Exon 3; lane 10: HLA-DPB1 Exon 2; lane 11: HLA-DQB1 Exon 2; lane 12: HLA-DRB1 Exon 2. M, DNA MW marker (250 bp–10 kb).

    Techniques Used: Extraction, Membrane, Amplification, Biomarker Discovery, Isolation, Agarose Gel Electrophoresis, Marker

    PCR of different HLA targets using gDNA prepared by PES membranes with different pore sizes. Buffy coat from the same sample was equally distributed and processed for gDNA extraction using either 0.22 µm (A) or 0.45 µm (B) PES membrane from EMD Millipore. Different targets of HLA loci were PCR amplified, as described in Validation of the gDNA isolation method using the PES membrane filter. PCR amplicons were analyzed on 2.5% agarose gel electrophoresis. Representative of a minimum of 4 independent experiments. Lanes 1–6: HLA-A Exon 2, HLA-A Exon 3, HLA-B Exon 2, HLA-B Exon 3, HLA-C Exon 2, and HLA-C Exon 3; lanes 7–9: Exon 3 genes of HLA-DPB1, DQB1, and DRB1; lanes 10–12: Exon 2 genes of DPB1, DQB1, and DRB1. M, DNA MW marker (250 bp–10 kb).
    Figure Legend Snippet: PCR of different HLA targets using gDNA prepared by PES membranes with different pore sizes. Buffy coat from the same sample was equally distributed and processed for gDNA extraction using either 0.22 µm (A) or 0.45 µm (B) PES membrane from EMD Millipore. Different targets of HLA loci were PCR amplified, as described in Validation of the gDNA isolation method using the PES membrane filter. PCR amplicons were analyzed on 2.5% agarose gel electrophoresis. Representative of a minimum of 4 independent experiments. Lanes 1–6: HLA-A Exon 2, HLA-A Exon 3, HLA-B Exon 2, HLA-B Exon 3, HLA-C Exon 2, and HLA-C Exon 3; lanes 7–9: Exon 3 genes of HLA-DPB1, DQB1, and DRB1; lanes 10–12: Exon 2 genes of DPB1, DQB1, and DRB1. M, DNA MW marker (250 bp–10 kb).

    Techniques Used: Extraction, Membrane, Amplification, Biomarker Discovery, Isolation, Agarose Gel Electrophoresis, Marker

    Agarose gel electrophoresis of gDNA samples digested with either HindIII or EcoRI. DNAs digested with HindIII (A–C); EcoR1 digested gDNAs (D–F). A, D) Buffy coat from the same sample was equally distributed and processed for gDNA extraction using either 0.22 µm PES membrane from EMD Millipore (lane 1) or QIAamp DNA Blood Mini Kit from Qiagen (lane 2). B, E) Buffy coat from 2 different samples was lysed independently, pooled together, and equally distributed and processed for gDNA extraction using the 0.22 µm PES membrane from different suppliers (lane 1: EMD Millipore; lane 2: Pall; lane 3: Sterlitech). C, F) Buffy coat from the same sample was equally distributed and processed for gDNA extraction using either the 0.22 µm (lane 1) or 0.45 µm (lane 2) PES membrane from EMD Millipore. M, DNA MW marker (75 bp–20 kb).
    Figure Legend Snippet: Agarose gel electrophoresis of gDNA samples digested with either HindIII or EcoRI. DNAs digested with HindIII (A–C); EcoR1 digested gDNAs (D–F). A, D) Buffy coat from the same sample was equally distributed and processed for gDNA extraction using either 0.22 µm PES membrane from EMD Millipore (lane 1) or QIAamp DNA Blood Mini Kit from Qiagen (lane 2). B, E) Buffy coat from 2 different samples was lysed independently, pooled together, and equally distributed and processed for gDNA extraction using the 0.22 µm PES membrane from different suppliers (lane 1: EMD Millipore; lane 2: Pall; lane 3: Sterlitech). C, F) Buffy coat from the same sample was equally distributed and processed for gDNA extraction using either the 0.22 µm (lane 1) or 0.45 µm (lane 2) PES membrane from EMD Millipore. M, DNA MW marker (75 bp–20 kb).

    Techniques Used: Agarose Gel Electrophoresis, Extraction, Membrane, Marker

    Related Articles

    Filtration:

    Article Title: Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood
    Article Snippet: membrane-based gDNA extraction method, we have isolated gDNAs from 29 additional human blood samples using the EMD Millipore 0.22 μm PES membrane. .. Estimation of the gDNA yield DNA concentrations of different samples were estimated using NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA). .. Assessment of the quality of gDNA preparations Agarose gel electrophoresis Integrity of gDNAs isolated in different sets of experiments was verified u

    Article Title: Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood
    Article Snippet: membrane-based gDNA extraction method, we have isolated gDNAs from 29 additional human blood samples using the EMD Millipore 0.22 μm PES membrane. .. Estimation of the gDNA yield DNA concentrations of different samples were estimated using NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA).

    Membrane:

    Article Title: Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood
    Article Snippet: membrane-based gDNA extraction method, we have isolated gDNAs from 29 additional human blood samples using the EMD Millipore 0.22 μm PES membrane. .. Estimation of the gDNA yield DNA concentrations of different samples were estimated using NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA). .. Assessment of the quality of gDNA preparations Agarose gel electrophoresis Integrity of gDNAs isolated in different sets of experiments was verified u

    Article Title: Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood
    Article Snippet: membrane-based gDNA extraction method, we have isolated gDNAs from 29 additional human blood samples using the EMD Millipore 0.22 μm PES membrane. .. Estimation of the gDNA yield DNA concentrations of different samples were estimated using NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA).

    Centrifugation:

    Article Title: Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood
    Article Snippet: membrane-based gDNA extraction method, we have isolated gDNAs from 29 additional human blood samples using the EMD Millipore 0.22 μm PES membrane. .. Estimation of the gDNA yield DNA concentrations of different samples were estimated using NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA). .. Assessment of the quality of gDNA preparations Agarose gel electrophoresis Integrity of gDNAs isolated in different sets of experiments was verified u

    Article Title: Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood
    Article Snippet: membrane-based gDNA extraction method, we have isolated gDNAs from 29 additional human blood samples using the EMD Millipore 0.22 μm PES membrane. .. Estimation of the gDNA yield DNA concentrations of different samples were estimated using NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA).

    Agarose Gel Electrophoresis:

    Article Title: Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood
    Article Snippet: membrane-based gDNA extraction method, we have isolated gDNAs from 29 additional human blood samples using the EMD Millipore 0.22 μm PES membrane. .. Estimation of the gDNA yield DNA concentrations of different samples were estimated using NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA). .. Assessment of the quality of gDNA preparations Agarose gel electrophoresis Integrity of gDNAs isolated in different sets of experiments was verified u

    Article Title: Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood
    Article Snippet: membrane-based gDNA extraction method, we have isolated gDNAs from 29 additional human blood samples using the EMD Millipore 0.22 μm PES membrane. .. Estimation of the gDNA yield DNA concentrations of different samples were estimated using NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA).

    Extraction:

    Article Title: Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood
    Article Snippet: membrane-based gDNA extraction method, we have isolated gDNAs from 29 additional human blood samples using the EMD Millipore 0.22 μm PES membrane. .. Estimation of the gDNA yield DNA concentrations of different samples were estimated using NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA). .. Assessment of the quality of gDNA preparations Agarose gel electrophoresis Integrity of gDNAs isolated in different sets of experiments was verified u

    Article Title: Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood
    Article Snippet: membrane-based gDNA extraction method, we have isolated gDNAs from 29 additional human blood samples using the EMD Millipore 0.22 μm PES membrane. .. Estimation of the gDNA yield DNA concentrations of different samples were estimated using NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA).

    Marker:

    Article Title: Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood
    Article Snippet: membrane-based gDNA extraction method, we have isolated gDNAs from 29 additional human blood samples using the EMD Millipore 0.22 μm PES membrane. .. Estimation of the gDNA yield DNA concentrations of different samples were estimated using NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA). .. Assessment of the quality of gDNA preparations Agarose gel electrophoresis Integrity of gDNAs isolated in different sets of experiments was verified u

    Article Title: Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood
    Article Snippet: membrane-based gDNA extraction method, we have isolated gDNAs from 29 additional human blood samples using the EMD Millipore 0.22 μm PES membrane. .. Estimation of the gDNA yield DNA concentrations of different samples were estimated using NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA).

    Amplification:

    Article Title: Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood
    Article Snippet: membrane-based gDNA extraction method, we have isolated gDNAs from 29 additional human blood samples using the EMD Millipore 0.22 μm PES membrane. .. Estimation of the gDNA yield DNA concentrations of different samples were estimated using NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA). .. Assessment of the quality of gDNA preparations Agarose gel electrophoresis Integrity of gDNAs isolated in different sets of experiments was verified u

    Article Title: Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood
    Article Snippet: membrane-based gDNA extraction method, we have isolated gDNAs from 29 additional human blood samples using the EMD Millipore 0.22 μm PES membrane. .. Estimation of the gDNA yield DNA concentrations of different samples were estimated using NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA).

    Biomarker Discovery:

    Article Title: Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood
    Article Snippet: membrane-based gDNA extraction method, we have isolated gDNAs from 29 additional human blood samples using the EMD Millipore 0.22 μm PES membrane. .. Estimation of the gDNA yield DNA concentrations of different samples were estimated using NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA). .. Assessment of the quality of gDNA preparations Agarose gel electrophoresis Integrity of gDNAs isolated in different sets of experiments was verified u

    Article Title: Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood
    Article Snippet: membrane-based gDNA extraction method, we have isolated gDNAs from 29 additional human blood samples using the EMD Millipore 0.22 μm PES membrane. .. Estimation of the gDNA yield DNA concentrations of different samples were estimated using NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA).

    Isolation:

    Article Title: Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood
    Article Snippet: membrane-based gDNA extraction method, we have isolated gDNAs from 29 additional human blood samples using the EMD Millipore 0.22 μm PES membrane. .. Estimation of the gDNA yield DNA concentrations of different samples were estimated using NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA). .. Assessment of the quality of gDNA preparations Agarose gel electrophoresis Integrity of gDNAs isolated in different sets of experiments was verified u

    Article Title: Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood
    Article Snippet: membrane-based gDNA extraction method, we have isolated gDNAs from 29 additional human blood samples using the EMD Millipore 0.22 μm PES membrane. .. Estimation of the gDNA yield DNA concentrations of different samples were estimated using NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA).



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    Image Search Results


    Flow chart depicting steps to assemble the filtration units and the methodology to be followed for isolating gDNA using the PES membrane. Note that the methodology described requires no centrifugation steps. RT, room temperature; TE, Tris-EDTA.

    Journal: Journal of Biomolecular Techniques : JBT

    Article Title: Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood

    doi: 10.7171/jbt.18-2902-001

    Figure Lengend Snippet: Flow chart depicting steps to assemble the filtration units and the methodology to be followed for isolating gDNA using the PES membrane. Note that the methodology described requires no centrifugation steps. RT, room temperature; TE, Tris-EDTA.

    Article Snippet: Estimation of the gDNA yield DNA concentrations of different samples were estimated using NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Filtration, Membrane, Centrifugation

    Agarose gel electrophoresis of gDNA samples prepared using different PES membranes or the QIAamp commercial kit. A) Buffy coat from the same blood sample was equally distributed and processed for gDNA extraction using either 0.22 µm PES membrane from EMD Millipore (lane 1) or QIAamp DNA Blood Mini Kit from Qiagen (lane 2). B) Buffy coat from different samples was lysed independently, pooled together, and equally distributed and processed for gDNA extraction using 0.22 µm PES membrane from different suppliers (lane 1: EMD Millipore; lane 2: Pall; and lane 3: Sterlitech). C) Buffy coat from the same sample was equally distributed and processed for gDNA extraction using either 0.22 µm (lane 1) or 0.45 µm (lane 2) PES membrane from EMD Millipore. M, DNA MW marker (75 bp–20 kb). Representative of a minimum of 4 independent experiments.

    Journal: Journal of Biomolecular Techniques : JBT

    Article Title: Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood

    doi: 10.7171/jbt.18-2902-001

    Figure Lengend Snippet: Agarose gel electrophoresis of gDNA samples prepared using different PES membranes or the QIAamp commercial kit. A) Buffy coat from the same blood sample was equally distributed and processed for gDNA extraction using either 0.22 µm PES membrane from EMD Millipore (lane 1) or QIAamp DNA Blood Mini Kit from Qiagen (lane 2). B) Buffy coat from different samples was lysed independently, pooled together, and equally distributed and processed for gDNA extraction using 0.22 µm PES membrane from different suppliers (lane 1: EMD Millipore; lane 2: Pall; and lane 3: Sterlitech). C) Buffy coat from the same sample was equally distributed and processed for gDNA extraction using either 0.22 µm (lane 1) or 0.45 µm (lane 2) PES membrane from EMD Millipore. M, DNA MW marker (75 bp–20 kb). Representative of a minimum of 4 independent experiments.

    Article Snippet: Estimation of the gDNA yield DNA concentrations of different samples were estimated using NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Agarose Gel Electrophoresis, Extraction, Membrane, Marker

    PCR of different HLA targets using gDNA prepared using the PES membrane or QIAamp kit. Buffy coat from the same blood sample was equally distributed and processed for gDNA extraction using either 0.22 µm PES membrane from EMD Millipore (A) or QIAamp DNA Blood Mini Kit from Qiagen (B). Different targets of HLA loci were PCR amplified, as described in Validation of the gDNA isolation method using the PES membrane filter. PCR amplicons were analyzed on 2.5% agarose gel electrophoresis. Representative of a minimum of 4 independent experiments. Lanes 1 and 2: HLA-A Exons 2 and 3; lanes 3 and 4: HLA-B Exons 2 and 3; lanes 5 and 6: HLA-C Exons 2 and 3; lanes 7 and 10: HLA-DPB1 Exons 3 and 2, respectively; lanes 8 and 11: HLA-DQB1 Exons 3 and 2, respectively; lanes 9 and 12: HLA-DRB1 Exons 3 and 2, respectively. M, DNA MW marker (250 bp–10 kb).

    Journal: Journal of Biomolecular Techniques : JBT

    Article Title: Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood

    doi: 10.7171/jbt.18-2902-001

    Figure Lengend Snippet: PCR of different HLA targets using gDNA prepared using the PES membrane or QIAamp kit. Buffy coat from the same blood sample was equally distributed and processed for gDNA extraction using either 0.22 µm PES membrane from EMD Millipore (A) or QIAamp DNA Blood Mini Kit from Qiagen (B). Different targets of HLA loci were PCR amplified, as described in Validation of the gDNA isolation method using the PES membrane filter. PCR amplicons were analyzed on 2.5% agarose gel electrophoresis. Representative of a minimum of 4 independent experiments. Lanes 1 and 2: HLA-A Exons 2 and 3; lanes 3 and 4: HLA-B Exons 2 and 3; lanes 5 and 6: HLA-C Exons 2 and 3; lanes 7 and 10: HLA-DPB1 Exons 3 and 2, respectively; lanes 8 and 11: HLA-DQB1 Exons 3 and 2, respectively; lanes 9 and 12: HLA-DRB1 Exons 3 and 2, respectively. M, DNA MW marker (250 bp–10 kb).

    Article Snippet: Estimation of the gDNA yield DNA concentrations of different samples were estimated using NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Membrane, Extraction, Amplification, Biomarker Discovery, Isolation, Agarose Gel Electrophoresis, Marker

    PCR of different HLA targets using gDNA prepared using PES membranes from different suppliers. Buffy coat from different samples was lysed independently, pooled together, and equally distributed and processed for gDNA extraction using the 0.22 µm PES membrane from different suppliers: EMD Millipore (A), Pall (B), and Sterlitech (C). Different targets of HLA loci were PCR amplified, as described in Validation of the gDNA isolation method using the PES membrane filter. PCR amplicons were analyzed on 2.5% agarose gel electrophoresis. Representative of a minimum of 4 independent experiments. Lane 1: HLA-A Exon 2; lane 2: HLA-A Exon 3; lane 3: HLA-B Exon 2; lane 4: HLA-B Exon 3; lane 5: HLA-C Exon 2; lane 6: HLA-C Exon 3; lane 7: HLA-DPB1 Exon 3; lane 8: HLA-DQB1 Exon 3; lane 9: HLA-DRB1 Exon 3; lane 10: HLA-DPB1 Exon 2; lane 11: HLA-DQB1 Exon 2; lane 12: HLA-DRB1 Exon 2. M, DNA MW marker (250 bp–10 kb).

    Journal: Journal of Biomolecular Techniques : JBT

    Article Title: Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood

    doi: 10.7171/jbt.18-2902-001

    Figure Lengend Snippet: PCR of different HLA targets using gDNA prepared using PES membranes from different suppliers. Buffy coat from different samples was lysed independently, pooled together, and equally distributed and processed for gDNA extraction using the 0.22 µm PES membrane from different suppliers: EMD Millipore (A), Pall (B), and Sterlitech (C). Different targets of HLA loci were PCR amplified, as described in Validation of the gDNA isolation method using the PES membrane filter. PCR amplicons were analyzed on 2.5% agarose gel electrophoresis. Representative of a minimum of 4 independent experiments. Lane 1: HLA-A Exon 2; lane 2: HLA-A Exon 3; lane 3: HLA-B Exon 2; lane 4: HLA-B Exon 3; lane 5: HLA-C Exon 2; lane 6: HLA-C Exon 3; lane 7: HLA-DPB1 Exon 3; lane 8: HLA-DQB1 Exon 3; lane 9: HLA-DRB1 Exon 3; lane 10: HLA-DPB1 Exon 2; lane 11: HLA-DQB1 Exon 2; lane 12: HLA-DRB1 Exon 2. M, DNA MW marker (250 bp–10 kb).

    Article Snippet: Estimation of the gDNA yield DNA concentrations of different samples were estimated using NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Extraction, Membrane, Amplification, Biomarker Discovery, Isolation, Agarose Gel Electrophoresis, Marker

    PCR of different HLA targets using gDNA prepared by PES membranes with different pore sizes. Buffy coat from the same sample was equally distributed and processed for gDNA extraction using either 0.22 µm (A) or 0.45 µm (B) PES membrane from EMD Millipore. Different targets of HLA loci were PCR amplified, as described in Validation of the gDNA isolation method using the PES membrane filter. PCR amplicons were analyzed on 2.5% agarose gel electrophoresis. Representative of a minimum of 4 independent experiments. Lanes 1–6: HLA-A Exon 2, HLA-A Exon 3, HLA-B Exon 2, HLA-B Exon 3, HLA-C Exon 2, and HLA-C Exon 3; lanes 7–9: Exon 3 genes of HLA-DPB1, DQB1, and DRB1; lanes 10–12: Exon 2 genes of DPB1, DQB1, and DRB1. M, DNA MW marker (250 bp–10 kb).

    Journal: Journal of Biomolecular Techniques : JBT

    Article Title: Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood

    doi: 10.7171/jbt.18-2902-001

    Figure Lengend Snippet: PCR of different HLA targets using gDNA prepared by PES membranes with different pore sizes. Buffy coat from the same sample was equally distributed and processed for gDNA extraction using either 0.22 µm (A) or 0.45 µm (B) PES membrane from EMD Millipore. Different targets of HLA loci were PCR amplified, as described in Validation of the gDNA isolation method using the PES membrane filter. PCR amplicons were analyzed on 2.5% agarose gel electrophoresis. Representative of a minimum of 4 independent experiments. Lanes 1–6: HLA-A Exon 2, HLA-A Exon 3, HLA-B Exon 2, HLA-B Exon 3, HLA-C Exon 2, and HLA-C Exon 3; lanes 7–9: Exon 3 genes of HLA-DPB1, DQB1, and DRB1; lanes 10–12: Exon 2 genes of DPB1, DQB1, and DRB1. M, DNA MW marker (250 bp–10 kb).

    Article Snippet: Estimation of the gDNA yield DNA concentrations of different samples were estimated using NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Extraction, Membrane, Amplification, Biomarker Discovery, Isolation, Agarose Gel Electrophoresis, Marker

    Agarose gel electrophoresis of gDNA samples digested with either HindIII or EcoRI. DNAs digested with HindIII (A–C); EcoR1 digested gDNAs (D–F). A, D) Buffy coat from the same sample was equally distributed and processed for gDNA extraction using either 0.22 µm PES membrane from EMD Millipore (lane 1) or QIAamp DNA Blood Mini Kit from Qiagen (lane 2). B, E) Buffy coat from 2 different samples was lysed independently, pooled together, and equally distributed and processed for gDNA extraction using the 0.22 µm PES membrane from different suppliers (lane 1: EMD Millipore; lane 2: Pall; lane 3: Sterlitech). C, F) Buffy coat from the same sample was equally distributed and processed for gDNA extraction using either the 0.22 µm (lane 1) or 0.45 µm (lane 2) PES membrane from EMD Millipore. M, DNA MW marker (75 bp–20 kb).

    Journal: Journal of Biomolecular Techniques : JBT

    Article Title: Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood

    doi: 10.7171/jbt.18-2902-001

    Figure Lengend Snippet: Agarose gel electrophoresis of gDNA samples digested with either HindIII or EcoRI. DNAs digested with HindIII (A–C); EcoR1 digested gDNAs (D–F). A, D) Buffy coat from the same sample was equally distributed and processed for gDNA extraction using either 0.22 µm PES membrane from EMD Millipore (lane 1) or QIAamp DNA Blood Mini Kit from Qiagen (lane 2). B, E) Buffy coat from 2 different samples was lysed independently, pooled together, and equally distributed and processed for gDNA extraction using the 0.22 µm PES membrane from different suppliers (lane 1: EMD Millipore; lane 2: Pall; lane 3: Sterlitech). C, F) Buffy coat from the same sample was equally distributed and processed for gDNA extraction using either the 0.22 µm (lane 1) or 0.45 µm (lane 2) PES membrane from EMD Millipore. M, DNA MW marker (75 bp–20 kb).

    Article Snippet: Estimation of the gDNA yield DNA concentrations of different samples were estimated using NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Agarose Gel Electrophoresis, Extraction, Membrane, Marker